Coding
ydeH

Part:BBa_K1019004:Design

Designed by: Boris Dyakov   Group: iGEM13_Toronto   (2013-09-16)


YdeH Diguanylate Cyclase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 775
    Illegal SapI.rc site found at 800


Design Notes

The gene was isolated using PCR and cloned into our expression vector pEBS where it is under inducible control via the lac promoter.


Source

E. coli K-12 MG1655

References